Infection and establishment of latency in the dog brain after direct inoculation of a nonpathogenic strain of herpes simplex virus-1

A number of diseases affecting the CNS occur in the dog and can be used as models for gene therapy in a large brain. HSV-1 has several potential advan tages as a Vector to transfer genes into the CNS. However, the ability of H SV-1 to infect CNS cells varies among species and no information was availa ble for the dog. When the nonpathogenic 1716 strain of HSV-1 was injected i nto the brains of normal dogs it established a latent infection without sig ns of pathology. Thus, it appears to be suitable as a vector for therapeuti c, or marker genes, in this species.