An improved method of ovule culture for producing interspecific hybrids inthe genus Lycoris (Amaryllidaceae)

Authors
Ma, B Tarumoto, I Liu, QC
Citation
B. Ma et al., An improved method of ovule culture for producing interspecific hybrids inthe genus Lycoris (Amaryllidaceae), J JPN S HOR, 70(4), 2001, pp. 460-462
Citations number
11
Categorie Soggetti
Plant Sciences
Journal title
JOURNAL OF THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE
ISSN journal
00137626 → ACNP
Volume
70
Issue
4
Year of publication
2001
Pages
460 - 462
Database
ISI
SICI code
0013-7626(200107)70:4<460:AIMOOC>2.0.ZU;2-L
Abstract
To improve the efficiency of embryo rescue in Lycoris, the components of ov ule culture medium, such as sucrose, pH and casein hydrolysate (CH), were s tudied by using selfed ovules of L, sanguinea as a model explant. A modifie d MS medium (mMS2) containing macro-salts adopted from Monnier's medium con taining 3% sucrose at pH 5.8 was found suitable for ovule culture in Lycori s. The addition of 500 mg.liter(-1) CH to mMS2 medium significantly promote d the germination and seedling formation rates and obtaining interspecific hybrids. Consequently, it was utilized in ovule culture for this purpose.