Identification of larval-stage antigens of Ascaris suum recognized with immune sera from pigs

Antigens were identified from the third-stage larvae (L3) and lung-stage la rvae of Ascaris suum by two-dimensional immunoblot method with antisera obt ained from pigs that received chemically abbreviated Ascaris suum larval in fections. Forty-seven and 13 spots were recognized as antigens from the L3 and lung-stage larvae, respectively. Their apparent molecular weight ranged from 20 to 101 kDa and their isoelectric point from 3.6 to 8.0. The presen t study provides a framework for further molecular cloning of those antigen s and consequently leads to the development of recombinant peptide vaccines against A. suum.