Induction of immune responses to glycoprotein gD of Aujeszky's disease virus with DNA immunization

In an attempt to produce a DNA vaccine to Aujesky's disease, the induction of immune responses against Aujesnky's disease virus (ADV) SD was investiga ted in mice. The plasmid was constructed by placing ADV go gene downstream of murine cytomegalovirus immediate early promoter of expression vector pMY K, which was injected twice on the skin of mice by using a gene-gun. All mi ce showed neutralizing antibodies against ADV go at 4 weeks after immunizat ion. The induction of cytotoxic T lymphocytes and splenic natural killer ce lls was also observed at 6 weeks post immunization. These results indicate that ADV go gene in the form of DNA vaccine may induce specific as well as non-specific immune responses in vivo.