Ropivacaine in the horse: its pharmacological responses, urinary detectionand mass spectral confirmation

This report evaluates the pharmacological responses, urinary detection and mass spectral confirmation of ropivacaine in horses. Ropivacaine, a potent local anesthetic (LA) recently introduced in human medicine, has an estimat ed highest no-effect dose (HNED) of about 0.4 mg/site as determined in our abaxial sesamoid block model. Apparent ropivacaine equivalents were detecta ble by ELISA screening using a mepivacaine ELISA test after administration of clinically effective doses. Mass spectral examination of postadministrat ion urine samples showed no detectable parent ropivacaine, but a compound i ndistinguishable from authentic 3-hydroxyropivacaine was recovered from the se samples. The study shows that ropivacaine is a potent LA in the horse, t hat clinically effective doses can be detected in postadministration sample s by ELISA-based screening, and that its major post administration urinary metabolite is 3-hydroxyropivacaine.