Optimization of submerged culture conditions for the mycelial growth and exo-biopolymer production by Cordyceps militaris

Aims: The objective of the present study was to determine the optimal cultu re conditions for exo-biopolymer production by Cordyceps militaris in shake flask culture. Methods and Results: The optimal temperature and initial pH for both myceli al growth and exo-biopolymer production by Cordyceps militaris in shake fla sk culture were found to be 20 degreesC and 6.0, respectively. Sucrose (40 g l(-1)) and corn steep powder (10 g l(-1)) were the most suitable carbon a nd nitrogen source for both mycelial growth and exo-biopolymer production. Conclusions: Under optimal culture conditions, the maximum exo-biopolymer c oncentration in a 5-1 jar fermenter indicated 10.3 g l(-1), which was appro ximately three times higher than that in shake flask culture. Significance and Impact of the Study: This process can have a significant i mpact on the industrial scale when sucrose and corn steep powder were used as carbon and nitrogen source.