SELECTIVE-INHIBITION OF GASTROINTESTINAL BETA-GLUCURONIDASE BY POLY(VINYLBENZYL D-GLUCARO(1,4)LACTONATE) .2. POLY(VINYLBENZYL D-GLUCARO(1,4) LACTONATE) IN-VITRO INHIBITION STUDIES

Authors
SACCO C CALABRESE EJ
Citation
C. Sacco et Ej. Calabrese, SELECTIVE-INHIBITION OF GASTROINTESTINAL BETA-GLUCURONIDASE BY POLY(VINYLBENZYL D-GLUCARO(1,4)LACTONATE) .2. POLY(VINYLBENZYL D-GLUCARO(1,4) LACTONATE) IN-VITRO INHIBITION STUDIES, Human & experimental toxicology, 13(11), 1994, pp. 759-763
Citations number
7
Categorie Soggetti
Toxicology
Journal title
Human & experimental toxicology → ACNP
ISSN journal
09603271
Volume
13
Issue
11
Year of publication
1994
Pages
759 - 763
Database
ISI
SICI code
0960-3271(1994)13:11<759:SOGBBP>2.0.ZU;2-T
Abstract
In vitro inhibition studies with beta-glucuronidase from purified E. c oli and mouse intestinal contents indicated that the polymer, poly(vin ylbenzyl D-glucaro(1,4)lactonate, is an effective beta-glucuronidase i nhibitor. Purified E. coli beta-glucuronidase was inhibited by 99.6% w ith 177 mM D-glucaro(1,4)lactone using the polymer-inhibitor. Similarl y, 95% inhibition of beta-glucuronidase activity of mouse intestinal c ontents was obtained with 177 mM and 50% inhibition was obtained with 31.5 mM D-glucaro(1,4)lactone based on the modified polymer. The struc tural requirements of an effective beta glucuronidase inhibitor based on the structure of the polymer-inhibitor are also discussed.