Sequence analysis of the 5.8S rDNA and ITS regions in evaluating genetic relationships among some species of Hypoxylon and related genera

Species belonging to Hypoxylon and related genera are bark pathogens and wo od decay agents of angiosperms, sometimes able to live asymptomatically in host tissues. In the present work the ITS regions of the ribosomal DNA from 21 isolates belonging to five species were sequenced and then compared wit h each other. A phylogenetic parsimony analysis among isolates was carried out. ITS2 appears to be the most informative among the sequenced portions o f rDNA. Results are strictly consistent with the latest taxonomic arrangeme nts obtained with classical morphological approaches. The ITS sequence anal ysis is an efficient and accurate method for discriminating among taxa of H ypoxylon and related genera.