A facile synthesis of 5 '-end solid-anchored, 3 '-end free oligodeoxyribonucleotides via the (5 '-> 3 ')-elongated phosphoramidite strategy

Authors
Sakakura, A Hayakawa, Y
Citation
A. Sakakura et Y. Hayakawa, A facile synthesis of 5 '-end solid-anchored, 3 '-end free oligodeoxyribonucleotides via the (5 '-> 3 ')-elongated phosphoramidite strategy, NUCLEOS NUC, 20(3), 2001, pp. 213-227
Citations number
30
Categorie Soggetti
Biochemistry & Biophysics
Journal title
NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS
ISSN journal
15257770 → ACNP
Volume
20
Issue
3
Year of publication
2001
Pages
213 - 227
Database
ISI
SICI code
1525-7770(2001)20:3<213:AFSO5'>2.0.ZU;2-N
Abstract
It is demonstrated that not only N-2- but also O-6-protection of the guanin e base is necessary for obtaining the oligodeoxyribonucleotides in high yie lds and at a high purity in the solid-phase synthesis via the (5 ' --> 3 ') -chain elongated phosphoramidite approach.