The production and evaluation of antibodies for enzyme immunoassay of AZTTP

Authors
Akeb, F Creminon, C Grassi, J Guedj, R Duval, D
Citation
F. Akeb et al., The production and evaluation of antibodies for enzyme immunoassay of AZTTP, NUCLEOS NUC, 20(3), 2001, pp. 243-250
Citations number
42
Categorie Soggetti
Biochemistry & Biophysics
Journal title
NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS
ISSN journal
15257770 → ACNP
Volume
20
Issue
3
Year of publication
2001
Pages
243 - 250
Database
ISI
SICI code
1525-7770(2001)20:3<243:TPAEOA>2.0.ZU;2-1
Abstract
We describe the development of the first enzyme immunoassay for quantifying AZTTP that does not use of radioactive labeling. Anti-AZTTP antibodies wer e raised in rabbits by immunizing with an AZTTP-kelhoyle limpet hemocyanin (KLH) conjugate. Competitive immunoassays indicated a nanomolar sensitivity to AZTTP. One of the antisera produced was specific for AZTTP.