Interleukin mRNA changes in mast cells stimulated by TSL-1 antigens

In this work we analyzed by RT-PCR, the mRNA changes for IL-4, IL-10, TNF a nd IFN ( induced by TSL-1 antigens in a rat mast cell line (HRMC) with muco sal characteristics. The data obtained showed an increase of 65 and 52 % in mRNA expression for IL-4 and TNF respectively and a decrease of 59 and 55 % in mRNAs for IFN gamma and IL-10. Our results suggest that TSI-I antigens induce the release from MC of regulatory molecules, such as IL-4 by an IgE independent mechanism. Our data also provides important information relate d to the ability of MC to participate not only in the effector phase agains t the infectious agents, but also in the orchestration of the immune respon se by the host against parasites.