M. Wick et al., ANTIGENIC CANCER-CELLS GROW PROGRESSIVELY IN IMMUNE HOSTS WITHOUT EVIDENCE FOR T-CELL EXHAUSTION OR SYSTEMIC ANERGY, The Journal of experimental medicine, 186(2), 1997, pp. 229-238
One enigma in tumor immunology is why animals bearing malignant grafts
can reject normal grafts that express the same nonself-antigen. An ex
planation for this phenomenon could be that different T cell clones re
act to the normal graft and the malignant cells, respectively, and onl
y the tumor-reactive clonotypes may be affected by the growing tumor.
To test this hypothesis, we used a T cell receptor transgenic mouse in
which essentially all CD8(+) T cells are specific for a closely relat
ed set of self-peptides presented on the MHC class I molecule L-d. We
iind that the tumor expressed L-d in the T cell receptor transgenic mi
ce but grew, while the L-d-positive skin was rejected. Thus, despite a
n abundance of antigen-specific T cells, the malignant tissue grew whi
le normal tissue expressing the same epitopes was rejected. Therefore,
systemic T cell exhaustion or anergy was not responsible for the grow
th of the antigenic cancer cells. Expression of costimulatory molecule
s on the tumor cells after transfection and preimmunization by full-th
ickness skin grafts was required for rejection of a subsequent tumor c
hallenge, but there was no detectable effect of active immunization on
ce the tumor was established. Thus, the failure of established tumors
to attract and activate tumor-specific T cells at the tumor site may b
e a major obstacle for preventive or therapeutic vaccination against a
ntigenic cancer.