Transfer of recombinant human granulocyte colony stimulating factor (rhG-CSF) from the maternal to the fetal circulation is not dependent upon a functional G-CSF-receptor

Administration of granulocyte colony stimulating factor (G-CSF), a haematop oietic growth factor, to pregnant rats increases neutrophil production in t he pugs. The mechanism for the placental transfer is unknown, but it has be en speculated to involve the placental G-CSF receptor (G-CSFR). The purpose of this study was to test that hypothesis. Pregnant mice were treated with a single subcutaneous dose of 50 mug/kg recombinant human G-CSF (rhG-CSF). Mice with an intact G-CSFR ('wild type',WT) and those with a homozygous de letion in the G-CSFR gene (G-CSFR deficient, 'knock-out', KO) were studied. At intervals after injection, fetuses were delivered and maternal blood, a mniotic fluid (AF) and fetal blood collected. G-CSF concentrations were mea sured using an enzyme linked immunosorbent assay specific for human G-CSF. Thirty minutes after injection, G-CSF was measurable in the AF (167 +/- 50 versus 445 +/- 217 pg/ml, mean +/- SEM, WT versus KO) and fetal plasma (774 +/- 673 versus 427 +/- 121 pg/ml, WT versus KO). Peak concentrations occur red 2 h after injection in WT dams (572 542 +/- 41 262 pg/ml) and 4 h in KO dams (616 100 +/- 96 300 pg/ml). Therefore, in mice, a functional G-CSFR i s not essential for the transfer of rhG-CSF from pregnant dams to their fet uses. (C) 2001 Harcourt Publishers Ltd.