It has not been possible to isolate a number of crucial enzymes involved in
plant cell wall synthesis. Recent progress in identifying some of these st
eps has been overcome by the isolation of mutants defective in various aspe
cts of cell wall synthesis and the use of these mutants to identify the cor
responding genes. Secondary cell walls offer numerous advantages for geneti
c analysis of plant cell walls. It is possible to recover very severe mutan
ts since the plants remain viable. In addition, although variation in secon
dary cell wall composition occurs between different species and between dif
ferent cell types, the composition of the walls is relatively simple compar
ed to primary cell walls. Despite these advantages, relatively few secondar
y cell wall mutations have been described to date. The only secondary cell
wall mutations characterised to date, in which the basis of the abnormality
is known, have defects in either the control of secondary cell wall deposi
tion or secondary cell wall cellulose or lignin biosynthesis. These mutants
have, however, provided essential information on secondary cell wall biosy
nthesis.