Antagonistic forces generated by myosin II and cytoplasmic dynein regulatemicrotubule turnover, movement, and organization in interphase cells

Citation
Amc. Yvon et al., Antagonistic forces generated by myosin II and cytoplasmic dynein regulatemicrotubule turnover, movement, and organization in interphase cells, P NAS US, 98(15), 2001, pp. 8656-8661
Citations number
32
Categorie Soggetti
Multidisciplinary
Journal title
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
ISSN journal
00278424 → ACNP
Volume
98
Issue
15
Year of publication
2001
Pages
8656 - 8661
Database
ISI
SICI code
0027-8424(20010717)98:15<8656:AFGBMI>2.0.ZU;2-5
Abstract
Photoactivation of caged fluorescent tubulin was used mark the microtubule (MT) lattice and monitor MT behavior in interphase cells, A broadening of t he photoactivated region occurred as MTs moved bidirectionally. MT movement was not inhibited when MT assembly was suppressed with nocodazole or Taxol ; MT movement was suppressed by inhibition of myosin light chain kinase wit h ML7 or by a peptide inhibitor. Conversely, MT movement was increased afte r inhibition of cytoplasmic dynein with the antibody 70.1. In addition, the half-time for MT turnover was decreased in cells treated with ML7, These r esults demonstrate that myosin II and cytoplasmic dynein contribute to a ba lance of forces that regulates MT organization, movement, and turnover in i nterphase cells.