Coupling of a supercritical fluid chromatography system to a hybrid (Q-TOF2) mass spectrometer: on-line accurate mass measurements

Citation
M. Garzotti et al., Coupling of a supercritical fluid chromatography system to a hybrid (Q-TOF2) mass spectrometer: on-line accurate mass measurements, RAP C MASS, 15(14), 2001, pp. 1187-1190
Citations number
5
Categorie Soggetti
Spectroscopy /Instrumentation/Analytical Sciences
Journal title
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
ISSN journal
09514198 → ACNP
Volume
15
Issue
14
Year of publication
2001
Pages
1187 - 1190
Database
ISI
SICI code
0951-4198(2001)15:14<1187:COASFC>2.0.ZU;2-N
Abstract
This note describes a simple and economical method to couple a supercritica l fluid chromatography (SFC) system (Berger Instruments, US) with a high-re solution hybrid mass spectrometer (Q-TOF 2; Micromass, UK). This experiment al arrangement has three distinct advantages: (1) coupling between the two systems can be effected without the need for an interface or hardware modif ications of either system, (ii) this experimental arrangement provides on-l ine accurate mass SFC/MS measurements which are indispensable for the chara cterisation of new chemical entities and unknown metabolites, and (iii) the characteristically fast spectral acquisition rate of the time-of-flight (T OF) analyser renders the present arrangement an important contribution to f uture semipreparative fraction collection setups which use mass spectrometr y as a detector. Copyright (C) 2001 John Wiley & Sons, Ltd.