A microelectrode array for real-time neurochemical and neuroelectrical recording in vitro

The development of a silicon neural recording device capable of measuring n eurochemical and neuroelectrical activity in tissue culture is described. I t consists of a microfabricated array of platinum and chloridized silver el ectrodes on a silicon substrate. A culture chamber affixed to the surface o f the device contains the culture medium and cells. Neuron cultures were vi able for over 75 days in the devices, generating both neuroelectrical and n eurochemical data in real-time. The resolution and speed of the device allo ws for the study of single neuron interactions with the ability to correlat e electrical signals directly with chemical responses. (C) 2001 Elsevier Sc ience B.V. All rights reserved.