Denitrification losses from irrigated sandy soil in Central Spain were stud
ied through laboratory and field experiments. In both cases, the acetylene
inhibition technique was applied. In the laboratory, potential denitrificat
ion was estimated by varying soil nitrate and carbon availability as well a
s oxygen depletion through the soil water content at a constant (25 degrees
C) temperature. Potential denitrification was evaluated within a range of 6
-12 kg N ha(-1) day(-1). When extra soil nitrate and carbon contents were n
ot supplied, observed potential denitrification ranged from 2 to 6 kg N ha(
-1) day(-1) depending on soil water content. The effect of temperature was
also studied with the aim of calibrating the influence of the day-night cyc
le on denitrification. A simple experiment was carried out indicating a N2O
+ N-2 emission four times greater in the case of treatment at a constant t
emperature than for treatment with a fluctuating temperature, but with the
same average temperature. Field experiments were carried out for 2 years on
two crops with different cycles: oats, grown in winter and spring with a s
hort irrigation period, and maize, an intensively irrigated summer crop. Mo
reover, three N treatments were tested: two controls with different N level
s and one fertilized with urea. Denitrification was periodically estimated,
and soil temperature, water and nitrate content simultaneously measured. D
aily denitrification rates (kg N ha(-1) day(-1)) ranged from O for all trea
tments to 1.2 for the urea-treated plot. Maximum emission was observed with
the urea treatment (40.7 kg N ha(-1)) during the whole period of the exper
iment; 10% of N applied with urea fertilizer was estimated to be lost, main
ly during the maize period, when high temperatures and soil N and water con
tents coincide. Finally, a prediction model was evaluated with acceptable r
esults. This makes it possible to evaluate agronomic practices in relation
to their effect on denitrification losses. (C) 2001 Elsevier Science Ltd. A
ll rights reserved.