Collagen binding by vaginal aggregative lactobacilli

Citation
I. Styriak et al., Collagen binding by vaginal aggregative lactobacilli, VET MED-CZ, 46(4), 2001, pp. 89-94
Citations number
23
Categorie Soggetti
Veterinary Medicine/Animal Health
Journal title
VETERINARNI MEDICINA
ISSN journal
03758427 → ACNP
Volume
46
Issue
4
Year of publication
2001
Pages
89 - 94
Database
ISI
SICI code
0375-8427(200104)46:4<89:CBBVAL>2.0.ZU;2-O
Abstract
Ten autoaggregating vaginal Lactobacillus strains (five of these strains we re selected among isolates from sows' vaginal swabs and the other five amon g isolates from cows' vaginal swabs) were investigated for their ability to bind type I collagen (Cn-I). All 10 autoaggregating strains in the range o f A(570nm) readings 0.118-1.806 bound to immobilised Cn-I (at concentration of 100 mug/ml) in wells of microtitre plates, however, Lactobacillus acido philus SV31 was much more adherent than the rest of the tested strains. The influence of culture medium on Cn-I binding was confirmed only in 50% of t he tested strains when agar-grown cells bound significantly more Cn-I than broth-grown cells. The specificity of the binding was confirmed since the C n-I binding by lactobacilli was abolished after their preincubation with th is protein. The effect of heparan sulphate and hyaluronic acid was tested o n 5 vaginal strains displaying the best Cn-I binding in microtitre plates a fter their cultivation on MRS agar plates. Both selected inhibitors signifi cantly (P < 0.001 or P < 0.01) reduced Cn-I binding by the majority of stra ins. The presence of the gene coding APF (aggregation-promoting factor) was detected in seven strains (all five sows' and two cows' Lactobacillus stra ins) by PCR.