3 '-Terminal sequence analysis of the RNA genome of the indian isolate of cardamom mosaic virus: A new member of genus macluravirus of potyviridae

Cardamom mosaic virus, a possible member of the family Potyviridae has been associated with the mosaic disease (Katte disease) of small cardamom in In dia. A virus isolated from the symptomatic cardamom leaves was positive in ELISA only with antiserum to the Guatemalan isolate of cardamom mosaic viru s and not with a number of other potyviruses. The size of the viral RNA (8. 5 kb) and the molecular weight of the coat protein (CP) (38 kDa) were deter mined. A 1.8-kb product containing the partial nuclear inclusion body (NIb) gene, the entire coat protein gene and the 3' untranslated region (UTR) wa s amplified by reverse transcription (RT) and polymerase chain reaction (PC R), cloned and sequenced. The viral origin of the clone was confirmed by No rthern hybridization with viral RNA. The experimentally determined N-termin al sequence of the CP matched with the deduced amino acid sequence of the C P gene. Sequence analysis of the clone suggests that the cardamom mosaic vi rus is a member of the Macluravirus genus of the family Potyviridae.