L. Subrahmanyan et al., Sequence variation and linkage disequilibrium in the human T-cell receptorbeta (TCRB) locus, AM J HU GEN, 69(2), 2001, pp. 381-395
Citations number
61
Categorie Soggetti
Research/Laboratory Medicine & Medical Tecnology","Molecular Biology & Genetics
The T-cell receptor (TCR) plays a central role in the immune system, and >9
0% of human T cells present a receptor that consists of the a TCR subunit (
TCRA) and the beta subunit (TCRB). Here we report an analysis of 63 variabl
e genes (BV), spanning 553 kb of TCRB that yielded 279 single-nucleotide po
lymorphisms (SNPs). Samples were drawn from 10 individuals and represent fo
ur populations-African American, Chinese, Mexican, and Northern European. W
e found nine variants that produce nonfunctional BV segments, removing thos
e genes from the TCRB genomic repertoire. There was significant heterogenei
ty among population samples in SNP frequency (including the BV-inactivating
sites), indicating the need for multiple-population samples for adequate v
ariant discovery. In addition, we observed considerable linkage disequilibr
ium (LD) (r(2) > 0.1) over distances of similar to 30 kb in TCRB, and, in g
eneral, the distribution of r(2) as a function of physical distance was in
close agreement with neutral coalescent simulations. LD in TCRB showed cons
iderable spatial variation across the locus, being concentrated in "blocks"
of LD; however, coalescent simulations of the locus illustrated that the h
eterogeneity of LD we observed in TCRB did not differ markedly from that ex
pected from neutral processes. Finally, examination of the extended genotyp
es for each subject demonstrated homozygous stretches of >100 kb in the loc
us of several individuals. These results provide the basis for optimization
of locuswide SNP typing in TCRB for studies of genotype-phenotype associat
ion.