Prediction of parameters c and a in reversed-phase high-performance liquidchromatography using retention parameters in gas liquid chromatography

The relationship of retention mechanism between gas liquid chromatography ( GLC) and reversed-phase high-performance liquid chromatography (RP-HPLC) is discussed. A model to predict the retention parameters c and a in retentio n equation in RP-HPLC using retention parameters in GLC is established, and it is verified by experimental data. By comparing the interaction forces i n GLC and RP-HPLC, it was found that the hydrogen-bonding interaction plays an important role in determining the retention mechanism of a solute in RP -HPLC, and the form of hydrogen-bonding is the eluent acting as hydrogen-bo nding donor acidity and the solute acting as hydrogen-bonding acceptor basi city. The-refractive force is important in both GLC and RP-HPLC, but the di pole moment interaction can be ignored.