Biological monitoring the exposure to polycyclic aromatic hydrocarbons of coke oven workers in relation to smoking and genetic polymorphisms for GSTM1 and GSTT1
Jhm. Van Delft et al., Biological monitoring the exposure to polycyclic aromatic hydrocarbons of coke oven workers in relation to smoking and genetic polymorphisms for GSTM1 and GSTT1, ANN OCCUP H, 45(5), 2001, pp. 395-408
Occupational exposure to polycyclic aromatic hydrocarbons (PAH) increases t
he risk of developing lung cancer. Human exposure Is often demonstrated by
increased internal levels of PAH metabolites and of markers for early biolo
gical effects, like DNA adducts and cytogenetic aberrations.
Objective: This study aimed to assess whether the current exposure to PAH o
f coke oven workers in a Dutch plant induced biological effects, and to det
ermine if these effects are influenced by tobacco smoking and by genetic po
lymorphisms for the glutathione S-transferase genes GSTM1 and GSTT1.
Methods: Urinary 1-hydroxypyrene (1-OHpyr) levels were used to monitor the
internal dose, while the internal effective dose was assessed by monitoring
PAH-DNA adducts, DNA strand breaks (Comet assay), sister-chromatid exchang
es (SCE) and cells with a high frequency of SCE (HFC) in lymphocytes togeth
er with micronuclei (MN) in exfoliated urothelial cells.
Results: Occupational exposure to PAH resulted in statistically significant
increased 1-OHpyr levels (P<0.001), but it did not cause a significant ind
uction of SCE, HFC, MN, DNA strand breaks or DNA adducts, Smoking caused a
significant increase of 1-OHpyr (P<0.05), SCE (P<0.001), HFC (P<0.001) and
DNA adducts (P<0.05), but not of MN or DNA strand breaks. Following correct
ion for the smoking-related effects, no occupational induction of the effec
t biomarkers could he discerned. Multi-variate analysis did not show a sign
ificant influence of GSTM1 and GSTT1 polymorphisms on any biomarker. Also n
o significant interactions were observed between the various biomarkers.
Conclusion: This study shows that in the examined plant, the occupational e
xposure to PAH does not result in measurable early biological effects (C) 2
001 British Occupational Hygiene Society. Published by Elsevier Science Ltd
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