Cloning and biochemical characterization of a class A beta-lactamase from Prevotella intermedia

Authors
Madinier, I Fosse, T Giudicelli, J Labia, R
Citation
I. Madinier et al., Cloning and biochemical characterization of a class A beta-lactamase from Prevotella intermedia, ANTIM AG CH, 45(8), 2001, pp. 2386-2389
Citations number
23
Categorie Soggetti
Microbiology
Journal title
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
ISSN journal
00664804 → ACNP
Volume
45
Issue
8
Year of publication
2001
Pages
2386 - 2389
Database
ISI
SICI code
0066-4804(200108)45:8<2386:CABCOA>2.0.ZU;2-I
Abstract
The gene encoding a B-lactamase of Prevotella intermedia was cloned and seq uenced. This gene, called cfxA2, shared 98% identity with cfxA, the structu ral gene of a beta -lactamase previously described in Bacteroides vulgatus. The deduced protein sequence had a K272E substitution. CfxA2 had the chara cteristics of class A, group 2e beta -lactamases.