Lc. Walker et al., Translocation (2;14) associated with complex rearrangements of the Ig heavy chain in non-Hodgkin lymphoma, CANC GENET, 128(2), 2001, pp. 137-140
Cytogenetic analysis of a patient with non-Hodgkin lymphoma revealed the fo
llowing karyotype: 49,XXX,t(2;14)(q21;q32),+4, +8,del(13)(q14q21), Southern
blot analysis with an Ig region probe showed non-productive rearrangements
indicative of a translocation involving the Ig locus. However, molecular c
loning of the abnormal rearrangements did not show novel sequences derived
from chromosome 2 but showed that the BCL-6 gene was juxtaposed to the IgH
enhancer. Three further clones with abnormal rearrangements involving the I
g locus, particularly Ig gamma3, were isolated. This suggests that the matu
re lymphoid cells, in this patient, were capable of undergoing indiscrimina
te switch cleavage and religation. (C) 2001 Elsevier Science Inc. All right
s reserved.