A very efficient method to cleave Lipid A and saccharide components in bacterial lipopolysaccharides

Authors
Manzo, E Molinaro, A Bedini, E De Castro, C Parrilli, M
Citation
E. Manzo et al., A very efficient method to cleave Lipid A and saccharide components in bacterial lipopolysaccharides, CARBOHY RES, 333(4), 2001, pp. 339-342
Citations number
10
Categorie Soggetti
Agricultural Chemistry","Chemistry & Analysis","Organic Chemistry/Polymer Science
Journal title
CARBOHYDRATE RESEARCH
ISSN journal
00086215 → ACNP
Volume
333
Issue
4
Year of publication
2001
Pages
339 - 342
Database
ISI
SICI code
0008-6215(20010719)333:4<339:AVEMTC>2.0.ZU;2-D
Abstract
A novel mild procedure for the selective cleavage of ketosidic linkages is developed using eerie ammonium nitrate (CAN) in anhydrous N,N-dimethylforma mide. Its application to lipopolysaccharides (LPS) is very significant beca use in the so far investigated LPS, the connection between the Lipid A regi on and the oligo(poly)saccharide part is always a keto-sugar. This procedur e has been tested on LPS of Escherichia coli which contains Kdo as a linker between Lipid A and OPS and on Acinetobacter haemoliticus which contains D -glyceroero-D-talo-2-octulopyranosonic acid (Ko) as a linker and it perform ed efficiently in both cases. (C) 2001 Elsevier Science Ltd. All rights res erved.