Optimization of HPLC conditions for the separation of complex crude mixtures produced in the synthesis of therapeutic peptide hormones

The solid phase peptide synthesis (SPPS) method is usually used for the syn thesis of new peptides of pharmaceutical interest. However, the final drug product commonly results in complex mixtures, where the target peptide must be separated from the undesired impurities. The linear salvation energy re lationships method (LSER) is used here in order to rapidly optimize the sep aration of the analytes present in the complex crude mixtures which are for med during the synthesis of peptide hormones with therapeutic interest, suc h as: carbetocin, eledoisin, leuprolide, goserelin and triptorelin. Resolut ion data for adjacent solute pairs may easily be predicted from these relat ionships. Separation under optimum conditions provides useful information a bout the purity of the target peptide and allows characterization of the mi xture components by LC-ES-MS. In addition conditions for the preparative pu rification of the candidates could be extrapolated from these analytical da ta.