Computer assisted scale up from analytical HPLC to preparative MPLC for the separation of phenolic compounds

A computer assisted method for the scale up of a column liquid chromatograp hic separation from the analytical to preparative scale is successfully pro posed to make the up-scaling process easier and faster. The test sample consisting of six phenolic compounds was chromatographed on an analytical HPLC column using two different gradient runs. Based on the chromatographic data achieved from these initial analyses, elution behaviou r of compounds could be simulated for alternative conditions using the DryL ab program. A simulative replacement of the analytical column with a prepar ative scale medium pressure LC (MPLC) column allowed the determination of g radient profile to allow sufficient separation in the preparative scale. A test run carried out in suggested simulated conditions matched well with ex pected elution times. Furthermore, this up-scaling procedure was successful ly applied to a plant extract.