Determination by high performance liquid chromatography of ornithine and lysine decaboxylases in sugar cane juices

Authors
Legaz, ME Fontaniella, B de Armas, R Vicente, C
Citation
Me. Legaz et al., Determination by high performance liquid chromatography of ornithine and lysine decaboxylases in sugar cane juices, CHROMATOGR, 53, 2001, pp. S260-S265
Citations number
32
Categorie Soggetti
Chemistry & Analysis","Spectroscopy /Instrumentation/Analytical Sciences
Journal title
CHROMATOGRAPHIA
ISSN journal
00095893 → ACNP
Volume
53
Year of publication
2001
Part
2
Supplement
S
Pages
S260 - S265
Database
ISI
SICI code
0009-5893(2001)53:<S260:DBHPLC>2.0.ZU;2-V
Abstract
Accurate and very sensitive HPLC methods which allow the simultaneous deter mination of the diamines putrescine (PUT) and cadaverine (CAD), resulting f rom ornithine decarboxylase (ODC) and lysine decarboxylase (LDC) activities or naturally occurring in sugarcane juices, are presented. Diamines were d etected as their dansyl derivatives by fluorescence emission. The correspon ding limits of detection were 21 ng mL(-1) for PUT and 5 ng mL(-1) for CAD. HPLC methods were compared with routine spectrophotometric assays.