Adsorption kinetics of human serum albumin on various types of chromatographic support

The adsorption of human serum albumin (HSA) on various types of chromatogra phic support (C6 reversed-phase, anion-exchanger, immunoadsorbent) was stud ied by analysis of frontal breakthrough curves. These curves are first char acterized by a sharp increase; a more diffuse profile is then observed at l onger elution times. Data were analyzed using a bi-Langmuir kinetic model a ssuming irreversible adsorption on two types of binding site. Fitting the m odel to the experimental data enables determination of apparent adsorption rates and the saturation capacities of both types of binding site.