Use of RP-HPLC on a dynamically coated artificial membrane to predict intestinal absorption of bile acids

Authors
Natalini, B Camaioni, E Colagioia, S Pellicciari, R
Citation
B. Natalini et al., Use of RP-HPLC on a dynamically coated artificial membrane to predict intestinal absorption of bile acids, CHROMATOGR, 53, 2001, pp. S453-S455
Citations number
20
Categorie Soggetti
Chemistry & Analysis","Spectroscopy /Instrumentation/Analytical Sciences
Journal title
CHROMATOGRAPHIA
ISSN journal
00095893 → ACNP
Volume
53
Year of publication
2001
Part
2
Supplement
S
Pages
S453 - S455
Database
ISI
SICI code
0009-5893(2001)53:<S453:UOROAD>2.0.ZU;2-B
Abstract
The preparation of an HPLC column with a non-covalent immobilized artificia l membrane, by dynamic coating of a RP-18 surface with phosphatidylcholine, is described. Passive transport of unconjugated bile acids has been correl ated with log k(w) values resulting from interaction with this biomembrane- like surface.