RP-HPLC for the determination of N-phenylhydroxylamine and related compounds

A simple, reversed-phase high performance liquid chromatographic method for the simultaneous determination of N-phenylhydroxylamine and related nitrog en-containing compounds, without poor peak asymmetries, is described. The a nalytes were detected at different wavelength depending on their absorbance maxima using a diode array detector after separation on an octadecyl-bonde d silica, Inertsil ODS-2, with aqueous potassium citrate buffered (pH 7) mo bile phase modified with methanol as organic solvent under gradient elution . The determined limits of quantification, measuring peak areas relative to an internal standard (N-tertbutyl-2-benzothiazolesulphenamide), are in the range of ng.mL(-1).