Sensitive micro-sample analysis of bisphenol A in serum by high-performance liquid chromatography with fluorescence detection

A rapid and sensitive HPLC method with fluorescence detection has been deve loped for the micro-sample analysis of bisphenol A in rat serum. Compounds were separated on a Spherisorb C-18 analytical column with acetonitrile-0.1 % triethylamine in deionized water, 51:46 (v/ v), as mobile phase. Bispheno l A was isolated by one-step extraction with dichloromethane. The calibrati on plot was linear(r = 0.999) over the concentration range 1-1000 ng mL(-1) . The limits of detection and quantitation were 0.2 ng mL(-1) and 1 ng mL(- 1), respectively, for 100-muL serum samples. The assay was successfully use d for a pharmacokinetic study after intravenous administration of a low dos e (0.1 mg kg(-1)) of bisphenol A to rats.