Abnormal balance in the angiopoietin-Tie2 system in human brain arteriovenous malformations

Brain arteriovenous malformations (BAVMs) are congenital vascular lesions t hat often present as cerebral hemorrhage in young adults. The variable natu re of the clinical course, especially with respect to spontaneous hemorrhag e, recurrence, growth, and regression, suggests that BAVMs are lesions with active angiogenesis and vascular remodeling. We examined mRNA and protein expression of angiopoietin 1 (Ang1) and Ang2 by semiquantitative reverse tr anscriptase-polymerase chain reaction, in situ hybridization, and Western b lot in BAVMs and control brains obtained from temporal lobectomy for medica lly intractable seizures. Although Ang1 mRNA levels were similar in BAVMs a nd controls, Ang1 protein levels were 30% lower in BAVMs than in controls. Ang2 mRNA levels were 40% higher and Ang2 protein levels were 8-fold higher in BAVMs than in controls. In situ hybridization showed that the Ang2 mRNA was localized to the perivascular area in BAVMs. This abnormal balance in the Ang-Tie2 system may, in part, explain the aberrant vascular phenotype i n BAVMs.