NOVEL MOUSE IGG-LIKE IMMUNOREACTIVITY EXPRESSED BY NEURONS IN THE MOTH MANDUCA-SEXTA - DEVELOPMENTAL REGULATION AND COLOCALIZATION WITH CRUSTACEAN CARDIOACTIVE PEPTIDE
Ka. Klukas et al., NOVEL MOUSE IGG-LIKE IMMUNOREACTIVITY EXPRESSED BY NEURONS IN THE MOTH MANDUCA-SEXTA - DEVELOPMENTAL REGULATION AND COLOCALIZATION WITH CRUSTACEAN CARDIOACTIVE PEPTIDE, Microscopy research and technique, 35(3), 1996, pp. 242-264
Immunoglobulin-related molecules have been shown to play important rol
es in cell-cell recognition events during the development of both vert
ebrate and invertebrate nervous systems. In the moth, Manduca sexta, w
e report the presence of novel, mouse, immunoglobulin G (mIgG)-like im
munoreactivity in a discrete population of identified neurosecretory n
eurons (the NS-Ls also known as the cell 27s) and interneurons (the IN
-704s). A number of polyclonal anti-mIgG antibodies were used to immun
ostain these cells in wholemount. The mIgG-like-immunoreactive (IR) ne
urons were present during embryogenesis through the developing adult s
tages, but disappeared in the postemerged adult. Biochemical analysis
of M. sexta ventral nerve cords revealed that the mIgG-like antigen is
a membrane-associated 27-kDa protein which is likely responsible for
the mIgG-like immunostaining observed. Unambiguous identification of t
he mIgG-like-IR neurons was based on neuronal morphology and our abili
ty to demonstrate conclusively that these neurons expressed immunoreac
tivity to an antiserum against crustacean cardioactive peptide (CCAP).
The NS-Ls and IN-704s were both shown to colocalize the CCAP and mIgG
-like immunoreactivities. The mIgG-like and CCAP-IR neurons were ident
ical to a subset of CCAP-IR neurons recently described by Davis et al.
[(1993) J. Comp. Neurol., 338:612-627] in pupae. We found these CCAP-
IR neurons, however, also to be present in larvae. The mIgG-like- and
CCAP-IR neurons included the NS-L pair of the subesophageal maxillary
neuromere, which projected anteriorly to the corpora cardiaca, and the
NS-L of the labial neuromere whose axons projected out the dorsal ner
ve of the next posterior ganglion. The mIgG-like and CCAP-IR NS-Ls wer
e also observed throughout the three thoracic ganglia, and all shared
strikingly similar structural features. These cells exited out the dor
sal nerve of the next posterior ganglion and eventually projected to t
he neurohemal release sites of the perivisceral organs. These neurons
appear to be the homologues of the abdominal CCAP-IR NS-Ls, neurons th
at in the adult switch their neurotransmitter and release the neuropep
tide bursicon. Our description of the distribution and developmental e
xpression of this novel mIgG-like immunoreactivity may provide new ins
ights into the regulation of neurotransmitter plasticity and/or recogn
ition-signaling events involved in the embryonic and postembryonic ass
embly of the nervous system. (C) 1996 Wiley-Liss, Inc.