Triclosan is a polychlorinated hydroxy diphenylether that has been widely u
sed as an antimicrobial compound. An enrichment using triclosan as a sole s
ource of carbon and energy yielded a consortium of bacteria capable of grow
ing on this compound. The dichloro ring was partially mineralized, resultin
g in the conversion of approximately 35% of the [C-14]triclosan to [C-14]CO
2. Use of molecular Fingerprinting: techniques and 16S rDNA cloning and seq
uencing aided in the identification and eventual isolation of an auxotrophi
c Sphingomonas-like organism, strain Rdl, which was able to partially miner
alize triclosan when grown on complex media. (C) 2001 Federation of Europea
n Microbiological Societies. Published by Elsevier Science B.V. All rights
reserved.