PURGING PERIPHERAL-BLOOD PROGENITOR-CELL GRAFTS FROM LYMPHOMA-CELLS -QUANTITATIVE COMPARISON OF IMMUNOMAGNETIC CD34(+) SELECTION SYSTEMS

Citation
U. Paulus et al., PURGING PERIPHERAL-BLOOD PROGENITOR-CELL GRAFTS FROM LYMPHOMA-CELLS -QUANTITATIVE COMPARISON OF IMMUNOMAGNETIC CD34(+) SELECTION SYSTEMS, Stem cells, 15(4), 1997, pp. 297-304
Citations number
32
Categorie Soggetti
Cell Biology","Biothechnology & Applied Migrobiology
Journal title
ISSN journal
10665099
Volume
15
Issue
4
Year of publication
1997
Pages
297 - 304
Database
ISI
SICI code
1066-5099(1997)15:4<297:PPPGFL>2.0.ZU;2-2
Abstract
Autologous peripheral blood progenitor cell (PBPC) transplantation is increasingly being used for treatment of indolent lymphomas, Since inv olvement of bone marrow and peripheral blood is frequent and methods t o reduce the lymphoma cell load of PBPC grafts are thus highly desirab le, we have studied purging of PBPC comparing two immunomagnetic CD34( +) selection systems (VarioMACS(TM), Miltenyi Biotech; Bergisch Gladba ch, Germany, and Isolex50(TM) System, Baxter; Irvine, CA), Samples of freshly collected mobilized PBPCs were contaminated with BALM-3 or KAR PAS422 lymphoma cells that had been labeled with the fluorescent DNA s tain Hoechst 33342., The mixture was subjected to separation with the two devices and the resulting ''CD34(+)'' fractions were screened for lyphoma cells by limiting dilution using fluorescence microscopy and b y polymerase chain reaction amplification of t(14;18) or CDRm-rearrang ements. Both devices yielded comparable purities (MACS 97% [87%-99%]; Isolex 97% [84%-99%]) and recoveries of CD34(+) cells (MACS 56% [30%-8 1%]; Isolex 45% [24%-63%]). The overall depletion of lymphoma cells wa s 3.9 log (2.6-5.9), however, residual contaminating cells were seen i n every single experiment, The purging efficacy was dependent on the t ype of contaminating lymphoma cell (BALM-3: 4.4 log [3.7-4.8]; KARPAS4 22: 3.2 log [2.6-4.2];p = 0.018), whereas the type of selection system used or the percentage of CD34(+) cells in the starting material had no influence, We conclude that excellent purification of CB34(+) cells leading to a vigorous depletion of lymphoma cells can be achieved wit h both CD34(+) selection systems investigated, However, the efficacy o f purging may greatly differ between individual lymphomas, and complet e eradication of contaminating cells from PBPC grafts may rarely be ac hieved with CD34(+) selection alone.