We have reported previously that a novel muscle cell growth factor, having
a structure of a peptide with sugar chains, was successfully purified from
porcine skeletal muscle. It was named s-myotrophin. To determine the role o
f s-myotrophin in skeletal muscle growth, the effect of s-myotrophin on pri
mary cultured chick skeletal muscle cells (composed almost totally of multi
nucleated myotubes) was investigated by comparing s-myotrophin with Insulin
-like growth factor-I (IGF-I). Both s-myotrophin and IGF-I significantly in
creased creatine kinase activity of the cultures; both substances gave simi
lar responses. Intracellar protein content was also increased by the additi
on of these factors. The content of myosin and actin in s-myotrophin treate
d culture in the differentiation medium was significantly higher than that
of the control (unstimulated). The content of those proteins in IGF-I treat
ed culture was also higher than that of control, but the differences were n
ot statistically significant. Immunoblot analysis confirmed that the amount
s of myosin and actin in the myocytes were greatly increased by s-myotrophi
n stimulation and also by IGF-I stimulation. Morphological observations usi
ng an anti-desmin antibody staining procedure demonstrated that the size of
both s-myotrophin and IGF-I treated myotubes was appreciably larger than t
hat of control myotubes. These results suggest that s-myotrophin is a poten
t mediator of skeletal muscle cell hypertrophy thorough the accumulations o
f muscle structural proteins. (C) 2001 Elsevier Science Ltd. All rights res
erved.