PREGNANCIES FROM SUBZONAL INSEMINATION OF UNFERTILIZED OOCYTES AFTER CONVENTIONAL IVF

Objective To evaluate the efficacy of subzonal insemination (SUZI) as applied to unfertilized oocytes after initial insemination. Methods Un fertilized oocytes at metaphase II obtained after conventional IVF fro m 14 patients were selected for reinsemination by SUZI. The fertilizat ion rate and pregnancy outcome were assessed. Results Of the 57 unfert ilized oocytes at metaphase II, 10 (17.5%) became fertilized after SUZ I. Eight of the 10 (80%) oocytes showed normal fertilization with two pronuclei while the other two (20%) oocytes were polyspermic. Five (35 .7%) of the 14 patients had embryo transfer resulting in two (14.3%) p regnancies. One patient had a spontaneous abortion and the other had a live birth at term. Conclusion Reinsemination of unfertilized oocytes by SUZI could be of value when fertilization fails after initial inse mination.