Analysis by HPLC of ryanodine and dehydroryanodine residues on fruits and in ryania powdery wood

A simple and rapid HPLC method to evaluate residues of the major ryanoids ( ryanodine and dehydroryanodine) on three fruits (olives, apples, and pears) has been developed. The pesticides were extracted from the fruits with hex ane and acetone solution (1:1, v/v). Cleanup was carried out with aminoprop yl-bonded silica cartridges. This method is characterized by recovery > 75% , precision < 11% RSD, and sensitivity of 0.020 mg/kg. The method can also be used to determine the level of active ingredients in ryania powdery wood .