CONTROL OF GLUCOSE-METABOLISM IN NEWBORN PIG ENTEROCYTES - EVIDENCE FOR THE ROLE OF HEXOKINASE

Citation
L. Posho et al., CONTROL OF GLUCOSE-METABOLISM IN NEWBORN PIG ENTEROCYTES - EVIDENCE FOR THE ROLE OF HEXOKINASE, Biochimica et biophysica acta. Molecular cell research, 1224(2), 1994, pp. 213-220
Citations number
46
Categorie Soggetti
Biology,Biophysics
ISSN journal
01674889
Volume
1224
Issue
2
Year of publication
1994
Pages
213 - 220
Database
ISI
SICI code
0167-4889(1994)1224:2<213:COGINP>2.0.ZU;2-Q
Abstract
The objective of the present work was to identify the regulatory step( s) in the post-natal development of a high glycolytic capacity previou sly evidenced in newborn pig enterocytes (Darcy-Vrillon et al. (1994) Pediat. Res., 36, 175-181. Glucose entry via the Na+/glucose cotranspo rter, estimated by the uptake of the non-metabolizable analogue methyl alpha-D-[U-C-14]glucopyranoside, slightly decreased between birth and 2 days of sucking. The flux of glucose metabolized into the pentose c ycle pathway slightly increased but could not account for the 3-fold i ncrease observed in the glycolytic capacity. Whereas the maximal activ ity of 6-phosphofructo-1-kinase did not change between stages, there w as a significant increase in hexokinase activity as well as in the flu x of glucose phosphorylated. These findings suggest that the stimulati on of glucose phosphorylation through hexokinase is the key event lead ing to an increased glycolytic capacity of small intestinal cells at t he onset of sucking.