C. Rouxel et al., Identification by SDS PAGE of green seaweeds (Ulva and Enteromorpha) used in the food industry, J APPL PHYC, 13(3), 2001, pp. 215-219
SDS PAGE was tested as an analytical tool for the identification of four gr
een algae (Ulva rigida, U. rotundata, Enteromorpha intestinalis, E. compres
sa) used as food ingredients. A reference pattern composed of the bands pre
sent all year long was performed for each species. The pattern for Ulva rot
undata consists of 7 bands located between 69.9 and 15.5 kDa with the prese
nce of triplicate bands at 29.5, 26.3 and 22.9 kDa. The pattern for Ulva ri
gida is consists of three bands with apparent molecular weights of 68.5, 56
.4 and 44.7 kDa. The Enteromorpha compressa pattern is characterised by six
bands located between 65.8 and 19.8 kDa. A double band with molecular weig
hts of 23.1 and 23.9 distinguished this pattern from the others. Six bands
situated between 66.4 and 19.4 kDa with a specific triplicate band of 25.9,
23.9 and 22.5 kDa constituted the specific pattern of Enteromorpha intesti
nalis. SDS PAGE appears to be suitable for the identification of green seaw
eed foods.