Identification by SDS PAGE of green seaweeds (Ulva and Enteromorpha) used in the food industry

Citation
C. Rouxel et al., Identification by SDS PAGE of green seaweeds (Ulva and Enteromorpha) used in the food industry, J APPL PHYC, 13(3), 2001, pp. 215-219
Citations number
12
Categorie Soggetti
Aquatic Sciences
Journal title
JOURNAL OF APPLIED PHYCOLOGY
ISSN journal
09218971 → ACNP
Volume
13
Issue
3
Year of publication
2001
Pages
215 - 219
Database
ISI
SICI code
0921-8971(200106)13:3<215:IBSPOG>2.0.ZU;2-6
Abstract
SDS PAGE was tested as an analytical tool for the identification of four gr een algae (Ulva rigida, U. rotundata, Enteromorpha intestinalis, E. compres sa) used as food ingredients. A reference pattern composed of the bands pre sent all year long was performed for each species. The pattern for Ulva rot undata consists of 7 bands located between 69.9 and 15.5 kDa with the prese nce of triplicate bands at 29.5, 26.3 and 22.9 kDa. The pattern for Ulva ri gida is consists of three bands with apparent molecular weights of 68.5, 56 .4 and 44.7 kDa. The Enteromorpha compressa pattern is characterised by six bands located between 65.8 and 19.8 kDa. A double band with molecular weig hts of 23.1 and 23.9 distinguished this pattern from the others. Six bands situated between 66.4 and 19.4 kDa with a specific triplicate band of 25.9, 23.9 and 22.5 kDa constituted the specific pattern of Enteromorpha intesti nalis. SDS PAGE appears to be suitable for the identification of green seaw eed foods.