The preparation of the chronic hyper-endotoxemia experimental animal modelby means of a drug delivery system

A drug delivery system (DDS) consisting of lipopolysaccharide (LPS) as a dr ug and 2-hydroxyethyl methacrylate (HEMA)-diethylene glycol dimethacrylate (2G) or -polyethylene glycol dimethacrylate (4G, 9G) copolymer was prepared , and used for the efficient preparation of an experimental animal model of chronic hyper-endotoxemia. The release profiles of LPS in the in-vitro tes t were greatly influenced by the composition of HEMA-2G, 4G, 9G in the copo lymer. It was found that LPS release from the DDS continued gradually and c onstantly throughout 2 weeks. In the in-vivo experiment with rats, the DDS maintained a high blood concentration level of LYS for 3 days. These result s strongly suggest the possibility of convenient and reproducible preparati on of a chronic hyper-endotoxemia animal model. (C) 2001 Elsevier Science B Y All rights reserved.