The relationship between retention, linear flow, and density difference incountercurrent chromatography

Countercurrent Chromatography is a high-resolution, liquid-liquid chromatog raphy process which is generic in nature and can be used with a wide range of solvent systems. This paper shows there are simple relationships between the operational variables affecting CCC, which lead to a clearer understan ding of how the process works. In the future, it will be easier to predict phase system behaviour from the physical characteristics of the phase syste m and the operating conditions of the coil planet centrifuge and, so, simpl ify the process of getting started with a new phase system.