Gh. Harris et P. Dal, Application of pH-zone-refining CCC to the isolation of antifungal fermentation products, J LIQ CHR R, 24(11-12), 2001, pp. 1775-1790
The application of pH-zone-refining countercurrent chromatography (CCC) to
the purification and bioassay guided isolation of novel antifungal agents f
rom fungal fermentation extracts is demonstrated with separations of three
families of acidic natural products. The separations were carried out using
two types of commercially available multilayer coil planet centrifuges. Us
ing this methodology as the final isolation step, three new glycosylated po
lyketides, arthrinosides A-C, were isolated from fermentation extracts of a
n Arthrinium sp. The three compounds differ in the length and hydroxylation
of the polyketide chain and were not easily separable using normal or reve
rse phase column chromatography. Mycoparasitic acids A and B, new natural p
roducts differing only in epoxidation of an endocyclic double bond, were se
parated directly from crude fungal culture extracts. This CCC method was ap
plied to the purification of a new biotransformation product of sordarin re
sulting from hydroxylation at C-11. Several hundred milligrams of this new
derivative were easily separated in a single chromatographic run.