P2X(3) knock-out mice reveal a major sensory role for urothelially released ATP

The present study explores the possible involvement of a purinergic mechani sm in mechanosensory transduction in the bladder using P2X(3) receptor knoc k-out (P2X(3)(-/-)) and wildtype control (P2X(3)(+/+)) mice. Immunohistoche mistry revealed abundant nerve fibers in a suburothelial plexus in the mous e bladder that are immunoreactive to anti-P2X(3). P2X(3)-positive staining was completely absent in the subepithelial plexus of the P2X(3)(-/-) mice, whereas staining for calcitonin gene-related peptide and vanilloid receptor 1 receptors remained. Using a novel superfused mouse bladder-pelvic nerve preparation, we detected a release of ATP proportional to the extent of bla dder distension in both P2X(3)(+/+) and P2X(3)(-/-) mice, although P2X(3)(- /-) bladder had an increased capacity compared with that of the P2X(3)(+/+) bladder. The activity of multifiber pelvic nerve afferents increased progr essively during gradual bladder distension (at a rate of 0.1 ml/min). Howev er, the bladder afferents from P2X(3)(-/-) mice showed an attenuated respon se to bladder distension. Mouse bladder afferents of P2X(3)(+/+), but not P 2X(3)(-/-), were rapidly activated by intravesical injections of P2X agonis ts (ATP or alpha, beta -methylene ATP) and subsequently showed an augmented response to bladder distension. By contrast, P2X antagonists [2', 3'- O-( 2,4,6- trinitrophenyl)-ATP and pyridoxal 5-phosphate 6-azophenyl-2', 4'-dis ulfonic acid] and capsaicin attenuated distension-induced discharges in bla dder afferents. These data strongly suggest a major sensory role for urothe lially released ATP acting via P2X(3) receptors on a subpopulation of pelvi c afferent fibers.