HISTAMINE DOES NOT POTENTIATE CYCLIC AMP-MEDIATED AMYLASE SECRETION IN THE GUINEA-PIG PANCREATIC ACINAR-CELLS

1. This study investigates the interaction between histamine and the a denylate cyclase systems involved in the secretion of amylase in isola ted guinea-pig pancreatic acinar cells. 2. Histamine caused dose relat ed enhancement of amylase release. Similarly, incubation of acini with increasing concentrations of vasoactive intestinal peptide (VIP) resu lted in a typical dose-dependent in crease in amylase output. 3. When pancreatic acinar cells were incubated with histamine in combination w ith VIP, amylase secretion did not differ statistically from secretion induced by histamine or VIP alone and was significantly lower than th eoretical additivity. Additionally, amylase secretion in the presence of histamine plus forskolin was significantly less than additive. The action of histamine was equally effective as VIP in causing cyclic ade nosine monophosphate (cAMP) increase. 4. These results indicate that h istamine may exert its secretory effects via the cyclic AMP pathway in the exocrine guinea-pig pancreas. GEN PHARMAC 29;2:211-215, 1997. (C) 1997 Elsevier Science Inc.