Videodensitometric HPTLC determination of fleroxacin, sparfloxacin, and cinoxacin in tablets

A new, simple, accurate, and rapid high-performance thin-layer chromatograp hic method has been developed for the determination of fleroxacin, sparflox acin, and cinoxacin in tablets. HPTLC was performed on silica gel 60 F-254 plates in horizontal chambers with dichloromethane-isopropyl alcohol-25% NH 3, 4 + 5 + 2, as mobile phase. Detection and quantification were achieved b y means of videodensitometry at lambda = 254 nm. The range of linearity was 0.08-0.48 mug muL(-1) for all the drugs analyzed. The sensitivity of detec tion was 10 ng spot(-1) for cinoxacin and 100 ng spot(-1) for sparfloxacin and fleroxacin. The relative standard deviations were <5.3%. The recovery o f the three antibiotics from tablets ranged from 96 to 105%.