M. Ceruti et al., Vinyl sulfide derivatives of truncated oxidosqualene as selective inhibitors of oxidosqualene and squalene-hopene cyclases, LIPIDS, 36(6), 2001, pp. 629-636
Various vinyl sulfide and ketene dithioacetal derivatives of truncated 2,3-
oxidosqualene were developed. These compounds, having the reactive function
s at positions C-2, C-15 and C-19 of the squalene skeleton, were studied as
inhibitors of pig liver and Saccharomyces cerevisiae oxidosqualene cyclase
s (OSC) (EC 5.4.99.7) and of Alicyclobacillus acidocaldarius squalene hopen
e cyclase (SHC) (EC 5.4.99.-). They contain one or two sulfur atoms in alph
a -skeletal position to carbons considered to be cationic during enzymatic
cyclization of the substrate and should strongly interact with enzyme nucle
ophiles of the active site. Most of the new compounds are inhibitors of the
OSC and of SHC, with Various degrees of selectivity. The methylthiovinyl d
erivative, having the reactive group at position 19, was the most potent an
d selective inhibitor of the series toward S. cerevisiae OSC, with a concen
tration inhibiting 50% of the activity of 50 nM, while toward the animal en
zyme it was 20 times less potent. These results could offer new insight for
the design of antifungal drugs.