Characterization of a canine parvovirus strain isolated from an adult dog

A CPV-2b strain was detected from an adult vaccinated dog, affected with se vere gastroenteritis. The faeces of the dog were positive to canine parvovi rus by a hemagglutination assay and gave a CPV-2b-like pattern by a hemaggl utination inhibition test using monoclonal antibodies. In vitro-cultivation of the virus was difficult and after a few passages on canine and feline cells, the presence of the virus was detectable only by an immunofluorescence assay on the feline cells, since hemagglutinating act ivity had disappeared. Characterization of the virus, by an indirect immuno fluorescence assay with monoclonal antibodies, confirmed the antigenic CPV- 2b-like pattern of the non-hemagglutinating virus.